Inhibitory Power Of Gotu Kola (Centella asiatica) Leave Extract Against Bacteria (Vibrio harveyii) In Vitro
https://doi.org/10.58466/lipida.v2i1.1411
Keywords:
Pegagan Leaf (C.asiatica), bioactive compound, V.harveyiiAbstract
Vibriosis disease is caused by Vibrio harveyii becteria attact. Use of antibiotics is prohibited because it has a negative impact on shrimp larvae because it will leave the residue in the body and cause resistence to V.harveyii. pegagan leaf (Centella asiatica) contains antibacterial active substances that can inhibit the growth of V.harveyii bacteria In Vitro. The purpose of this study is to determine the effectiveness of leaf extract of C.asiatica can inhibit thegrowth of V.harveyii bacteria In Vitro. The antibacterial test was performed by disc diffusion method, which consisted of 5 treatments, 5% 8% and 10% and 2 control, is positive control (Tetracycline 0,01%) and negative control (aquades). The result of secondary metabolite compound of C.asiatica extract was analyzedqualitively. In leaf extract of gotu kola contains bioactive compounds such as alkaloids, phenols, saponins and tannins. In the antibacterial test of C.asiatica extract was able to inhibit the growth of bacteria with the average diameter of inhibit zone formed from each concentration of extract that is 5% = 14,16 mm concentration 8% = 16,63 mm concentration 10% = 21,44. From the result, obtained leaf extract of C.asiatica showed the abilityto inhibit the growth of V.harveyii bacteria close to the drag in the positive control (tetracycline).
References
Awaludin dan Rahmat 2015. Analisis Kematangan Gonad Induk Udang Putih (Litopenaeuns vannamei) Setelah Pemberian Ekstrak Etanol Karamunting (Melastoma malabathricum). Tesis. SITH ITB.
Baticados, M.C.L., F.R. Cruz-Lacierda, M.C. de la Crus, R.C. Duremdes, Fernandez, R. Rgacutan, C.R. Lavilla-Pitogo, and G.D. Lio-Po. 1990. Diseases of penaid shrimp in the Philippines. Aquaculture Extension Manual No. 16 May 1990. SEAFDEC. 46 p.
Dash BK, Faruquee HM, Biswas SK, Alam MK, Sisir SM. Prodhan UK. Antibacterial and Antifungal Actifities of Severai Extracts of Centella asiatica L. Against Some Human Pathogenic Microbes. Life Sciences and Madicine Research. 2011; 2011: LSMR-35.
Departemen Kesehatan RI. (1995). Farmakope Indonesia Edisi IV. Jakarta: Departemen Kesehatan RI. Hal. 1033.
Lavila-Pitogo, C.R., E.M. Leano, and M.G. Paner. 1998. Aquaculture. 164. Lavila-Pitogo, C.R., E.M. Leano, and M.G. Paner. 1998. Aquaculture. 164. 337-349.
Harborne, J.B., (1987), Metode fitokimia, Edisi ke dua, ITB, Bandung.
Karou D et al. 2005. Antibacterial activity of alkaloids from sida acuta. African J Biotechnology 4:1452-1457.
Volk dan Wheeler. 1984. Mikrobiologi Dasar Edisi Kelima Jilid I. Erlangga. Jakarta.
Sudira, IW, Merdana, IM & Wibawa, IP, 2011, “Uji daya hambat ekstrak daun kedondong (Lanneagrandis Engl) terhadap pertumbuhan bakteri Erwiia Carotovora’, Buletin Veteriner Udayana, vol. 3, no, 1, hal. 45-50.
Farkas, J. dan Malik, S.E. 1986. Vibrio Disease of sheatfish (Silurus sp). Aquaculture. 24 : 81-88
Egidius, E. 1987. Vibriosis. Pathogenicity and Pathology. A Review. Aquaculture: 87: 15-28.
Tedja I,S (2012), Mikrobioligi Esensial.
Jawestz, E., Melnick, J.L. and Adelberg, E.A. 1984. Review of Medical Microbiology. Lange Medical Publication. California. 34 : 56-67
Pelezar, M.J., Chan E. C. S., 1988. Dasar-dasar Mikrobiologi. Jakarta: Universitas Indonesia Press.
Poeloengan, M., Andriani., M.N Susan., I. Komala dan M. Hasnita 2007. Uji Daya Antibakteri Ekstrak Etanol Kulit Batang Bungur (Lagerstroemis speciosa)
